Product ID:4312524, CRYSTAL VIOLET (GRAM STAIN KIT)
MSDS Date:07/28/1998
FSC:6550
NIIN:01-314-2719
Status Code:A
MSDS Number: CKLVP

BECTON DICKINSON MICROBIOLOGY SYSTEMS

250 SCHILLING CIRCLE
COCKEYSVILLE, MD 21030
US

Emergency Phone: 410-771-0101

Info Phone: 410-771-0101

Cage: 05545

FISHER SCIENTIFIC CO. CHEMICAL MFG DIV

FAIRLAWN, NJ 07410-2802 US

Telephone: 201-796-7100

Cage: 1B464

ISOPROPANOL

CAS: 67-63-0

RTECS: NT8050000

OSHA PEL 980 MG/M3;400 PPM

ACGIH TLV: 983 MG/M3;400 PPM

ETHANOL

CAS: 64-17-5

RTECS: KQ6300000

OSHA PEL 1900 MG/M3;1000 PPM

ACGIH TLV: 1880 MG/M3;1000 PPM

CRYSTAL VIOLET

CAS: 548-62-9

RTECS: BO9000000

LD50 LC50 Mixture:NO DATA GIVEN ON PRODUCT AS WHOLE
Health Hazards Acute and Chronic:ON THE SKIN: IRRITANT TO SKIN AND
MUCOUS MEMBRANES. ON THE EYE: IRRITATING EFFECT. SENSITIZATION: NO
SENSITIZING EFFETS KNOWN. TARGET ORGANS: THYROID, KIDNEY, URETER OR
BLADDER TUMORS.
Explanation of Carcinogenicity:CARCINOGENIC.
Effects of Overexposure:ON THE SKIN: IRRITANT TO SKIN AND MUCOUS
MEMBRANES. ON THE EYE: IRRITATING EFFECT. SENSITIZATION: NO
SENSITIZING EFFETS KNOWN.
Medical Cond Aggravated by Exposure:NO DATA PROVIDED BY RESPONSIBLE
PARTY

First Aid:INHALATION: SUPPLY FRESH AIR. IF REQUIRED, GIVE ARTIFICIAL
RESPIRATION. KEEP PATIENT WARM. SEEK MEDICAL ADVICE. IN CASE OF
UNCONSCIOUSNESS, PLACE PATIENT ON SIDE POSITION FOR TRANSPORTATION.
SKIN CONT ACT: IMMEDIATELY WASH WITH SOPA AND WATER. RINSE
THOROUGHLY. EYE CONTACT: RINSE OPENED EYE FOR SEVERAL MINUTES UNDER
RUNNING WATER. THEN CONSULT A DOCTOR. SWALLOWING: IMMEDIATELY CALL
A DOCTOR. INFORM ATION FOR DOCTOR: SHOW PRODUCT LABEL.

Autoignition Temp:Autoignition Temp Text:NONE
Extinguishing Media:CARBON DIOXIDE (CO2), EXTINGUISHING POWDER OR WATER
SPRAY. FIGHT LARGER FIRES WITH WATER OR ALCOHOL RESISTANT FOAM.
Fire Fighting Procedures:WEAR SLEF-CONTAINED RESPIRATORY PROTECTIVE
DEVICE. WEAR FULL PROTECTIVE SUIT.
Unusual Fire/Explosion Hazard:PRODUCT DOES NOT PRESENT AN EXPLOSION
HAZARD.

Spill Release Procedures:WEAR PROTECTIVE EQUIPMENT. KEEP UNPROTECTED
PERSONS AWAY. WIPE UP WITH DAMP SPONGE OR MOP. ENSURE ADEQUATE
VENTILATION.
Neutralizing Agent:NO DATA PROVIDED BY RESPONSIBLE PARTY

Handling and Storage Precautions:ENSURE GOOD VENTILATION/EXHAUST AT THE
WORKPLACE. KEEP IGNITION SOURCES AWAY. DO NOT SMOKE.
Other Precautions:STORAGE TEMPERATURE: 15-30C.

Respiratory Protection:IN CASE OF BRIEF EXPOSURE, USE A CHEMICAL FUME
HOOD OR A NIOSH/MSHA-APPROVED RESPIRATORY PROTECTIVE DEVICE. IN
CASE OF INTENSIVE OR LONGER EXPOSURE USE A RESPIRATORY PROTECTIVE
DEVICE THAT IS INDEPEN DENT OF CIRCULATING AIR.
Ventilation:CHEMICAL FUME HOOD.
Protective Gloves:PROTECTIVE GLOVES.
Eye Protection:SAFETY GLASSES.
Other Protective Equipment:BODY PROTECTIVE WORK CLOTHING (LAB COAT).
Work Hygienic Practices:WASH HANDS BEFORE BREAKS AND AT THE END OF
WORK. AVOID CONTACT WITH SKIN AND EYES. IMMEDIATELY REMOVE ALL
SOILED AND CONTAMINATED CLOTHES. KEEP AWAY FROM FOODSTUFFS,
BEVERAGES AND FEED.
Supplemental Safety and Health
NO DATA PROVIDED BY RESPONSIBLE PARTY

HCC:T6
Boiling Pt:B.P. Text:UNDETERMINED.
Melt/Freeze Pt:M.P/F.P Text:UNDETERMINED.
Appearance and Odor:FORM LIQUID, COLOR VIOLET, ODOR CHARACTERISTIC.

NO DANGEROUS REACTIONS KNOWN.
Stability Condition to Avoid:NO DECOMPOSITION IF USED ACCORDING TO
SPECIFICATIONS.
Hazardous Decomposition Products:NITROGEN OXIDES, CARBON MONOXIDE,
CARBON DIOXIDE AND AMMONIA.

Waste Disposal Methods:MUST NOT BE DISPOSED OF WITH SOLID WASTE. MUST
ADHERE TO STATE AND FEDERAL REGULATIONS. DISPOSAL MUST BE MADE
ACCORDING TO THE REGULATIONS FOUND IN 40 CFR 261. THIS PRODUCT IS
NOT CONSIDERED A RCRA HA ZARDOUS WASTE. FOLLOW LOCAL AND STATE
REGULATIONS. CLEANSING AGENT: WATER, IF NECESSARY WITH CLEANSI NG
AGENTS.

Toxicological Information:ACUTE TOXICITY: 64-17-5, ETHANOL-ORAL: LD50:
7060 MG/KG (RAT); INHALATIVE: LC50.4 HR: 20000 MG/L (RAT).
548-62-9, CRYSTAL VIOLET-ORAL: LD50: 96 MG/KG (MOU). 67-63-0,
ISOPROPANOL-ORAL: LD50: 4570 MG/KG , DERMAL: LD50: 13400 MG/KG
(RABBIT), INHALATIVE: LC50/4 HR: 30 MG/L (RAT). ON THE SKIN:
IRRITANT TO SKIN AND MUCOUS MEMBRANES. ON THE EYE: IRRITATING
EFFECT. SENSITIZATION: NO SENSITIZING EFFETS KNO WN. TARGET ORGANS:
THYROID, KIDNEY, URETER OR BLADDER TUMORS. ADDITIONAL TOXICOLOGICAL
INFORMATION: IRRITANT. CARCINOGENIC.

Disclaimer (provided with this information by the compiling agencies):
This information is formulated for use by elements of the Department
of Defense. The United States of America in no manner whatsoever,
expressly or implied, warrants this information to be accurate and
disclaims all liability for its use. Any person utilizing this
document should seek competent professional advice to verify and
assume responsibility for the suitability of this information to their
particular situation.

Ecological:THE ECOLOGICAL EFFECTS HAVE NOT BEEN THOROUGHLY
INVESTIGATED, BUT CURRENTLY NONE HAVE BEEN IDENTIFIED. WATER HAZARD
CLASS 1: SLIGHTLY HAZARDOUS FOR WATER.

Transport Information:DOT REGULATIONS/HAZARD CLASS: NO DATA PROVIDED BY
RESPONSIBLE PARTY. LAND TRANSPORT ADR/RID (CROSS-BORDER): NO DATA
PROVIDED BY RESPONSIBLE PARTY. MARITIME TRANSPORT IMDG/IMDG CLASS:
NO DATA PROVIDED BY RESPONSIBLE PARTY. MARINE POLLUTANT: NO.
AIR-TRANSPORT ICAO-TI AND IATA-DGR/ICAO/IATA CLASS: NO DATA
PROVIDED BY RESPONSIBLE PARTY.

SARA Title III Information:ETHANOL: SARA 311/312: NOT SUBJECT TO
REPORTING. SARA 313: NOT SUBJECT TO REPORTING. ISOPROANOL: SARA
311/312: NOT SUBJECT TO REPORTING. SARA 313: MAY BE SUBJECT TO
REPORTING.
Federal Regulatory Information:CERCLA: THIS PRODUCT CONTAINS ETHANOL
AND HAS A REPORTING QUANTITY (RQ) OF 5000 LBS. TSCA: THIS PRODUCT
IS EXEMPT UNDER TSCA. CERCLA: THIS PRODUCT CONTAINS ISOPROPANOL AND
HAS A REPORTING QUANTITY (RQ ) OF 100 LBS. TSCA: THIS PRODUCT IS
EXEMPT UNDER TSCA. TECHNICAL INSTRUCTIONS (AIR): CLASS: III - 9.8%.
State Regulatory Information:NO DATA PROVIDED BY RESPONSIBLE PARTY